Description

Starting in 2010, the Field Seed Program began in North Dakota, requiring variety identification (VID) for certification of all seed lots of hard red spring wheat, barley, and field pea. In conjunction with the North Dakota State Seed Department (NDSSD), NAGC has assumed all VID testing for the state of North Dakota. With most new seed varieties protected by plant variety protection (PVP) laws, VID testing has become a valuable tool in PVP enforcement. The NDSSD uses VID testing during the regulatory inspection process to verify the stated variety on labels matches what is in the container/bin. Variety ID testing is required for all lots of barley for final certification in North Dakota.

NAGC’s CropFiler variety identification testing is conducted using specific DNA simple sequence repeats (SSRs) panels. Seed DNA is extracted from each sample and then subjected to polymerase chain reaction (PCR) using the specific SSR panels for barley, chickpea, durum, field pea, or wheat. The PCR products are then subjected to capillary electrophoresis (CE) and a sample profile is obtained. The sample profile is used to search NAGC’s CropFiler database to assign a variety to the sample.

NAGC’s current list of barley controls that have been profiled with BarleyFiler are listed below. If a control isn’t listed, feel free to reach out to us at 701.239.1451 to discuss profiling a new control (it’s free!).

This project was funded in part by the North Dakota Department of Agriculture Bioscience Innovative Grant, North Dakota State Seed Department, North Dakota Wheat Commission, and American Malting Barley Association.