Description

Protein variety testing is conducted using native polyacrylamide gel electrophoresis (PAGE). This method in which an electrical current is applied to the gel, causing the proteins to separate based on charge. The patterns of protein banding are based on genetic composition and is to be considered the ‘fingerprint’ for that specific variety, are visualized using a stain. Known variety controls are run in conjunction with unknown samples and compared to each other for identification (Cooke, 1992).

NAGC currently runs oat and wheatgrass on PAGE gels. NAGC has limited controls for these crops, contact us at 701.239.1451 or at research@genotypingcenter.com to see if you need to provide a control sample with the sample to be tested.

References

Cooke, R. J. 1992. Variety Testing: Electrophoresis Testing, Cambridge, UK, International Seed Testing Association.